![SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure](https://cdn.numerade.com/ask_images/ac81f6690f8a4daaa48795d147bc881b.jpg)
SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure
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A tailed PCR procedure for cost‐effective, two‐order multiplex sequencing of candidate genes in polyploid plants - Gholami - 2012 - Plant Biotechnology Journal - Wiley Online Library
![SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure](https://cdn.numerade.com/ask_previews/d85c4d-d3b-f008-56d6-57236b440de6_large.jpg)
SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure
![Noncontinuously Binding Loop-Out Primers for Avoiding Problematic DNA Sequences in PCR and Sanger Sequencing - ScienceDirect Noncontinuously Binding Loop-Out Primers for Avoiding Problematic DNA Sequences in PCR and Sanger Sequencing - ScienceDirect](https://ars.els-cdn.com/content/image/1-s2.0-S1525157814001032-gr2.jpg)
Noncontinuously Binding Loop-Out Primers for Avoiding Problematic DNA Sequences in PCR and Sanger Sequencing - ScienceDirect
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Primer and amplicon construction. The first round of PCR uses a forward... | Download Scientific Diagram
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An alternative genotyping method using dye-labeled universal primer to reduce unspecific amplifications | Molecular Biology Reports
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